Human IL-6 Recombinant Rabbit Monoclonal Antibody [PS00-21] – BSA and Azide Free (Capture)

The Human IL-6 Recombinant Rabbit Monoclonal Antibody [PS00-21] is a capture antibody designed for research applications involving human Interleukin-6 (IL-6). This antibody is supplied in a BSA-free and azide-free formulation and is intended for use in sandwich ELISA assays to quantify human IL-6 in biological samples.

What Is Interleukin-6 (IL-6)?

Interleukin-6 (IL-6) is a multifunctional cytokine involved in immune regulation, inflammation, hematopoiesis, and tissue homeostasis. It is produced by several cell types, including macrophages, monocytes, fibroblasts, endothelial cells, and activated immune cells.

IL-6 plays an important role in the body's response to infection and tissue injury. It acts as a pro-inflammatory cytokine during immune activation and also participates in regulatory processes that help control inflammatory responses.

The IL-6 protein is particularly important in the acute-phase response, where it stimulates the liver to produce acute-phase proteins such as C-reactive protein (CRP). It also contributes to fever, immune-cell differentiation, and the regulation of inflammatory signaling pathways.

Biological Functions of IL-6

IL-6 Signaling Pathways

IL-6 activates cellular responses through interactions with the IL-6 receptor (IL-6R) and the signal-transducing protein gp130. This signaling system regulates several intracellular pathways involved in inflammation, immune responses, and cellular survival.

Classical IL-6 Signaling

In classical signaling, IL-6 binds to membrane-bound IL-6 receptors. The resulting complex associates with gp130 and activates intracellular signaling pathways, including JAK/STAT signaling.

Trans-Signaling

In trans-signaling, IL-6 binds to soluble IL-6 receptor (sIL-6R). This complex can interact with gp130 on cells that do not express membrane-bound IL-6R, expanding the range of cells responsive to IL-6.

Both signaling mechanisms are important research subjects in immunology, inflammatory disease studies, and therapeutic development.

What Is a Capture Antibody in Sandwich ELISA?

A capture antibody is an antibody used to immobilize a specific target protein during a sandwich enzyme-linked immunosorbent assay (ELISA). It is typically coated onto the surface of a microplate, where it selectively binds the target antigen from a biological sample.

For human IL-6 measurement, the capture antibody recognizes IL-6 and retains it on the assay surface. A second antibody, known as the detection antibody, binds to another accessible epitope of the captured protein.

Recommended Antibody Pair for Human IL-6 ELISA

The recommended sandwich ELISA antibody combination includes the following capture and detection reagents:

The detection antibody HA721168 is supplied in an unconjugated format. The two antibodies are recommended as a pair for sandwich ELISA quantification of human IL-6.

Why Choose a BSA and Azide-Free Antibody?

Bovine serum albumin (BSA) and sodium azide are commonly used as stabilizing or preservative components in antibody formulations. However, some laboratory applications require antibody preparations without these additional substances.

Research Applications of Human IL-6 Antibody

1. Inflammation Research

IL-6 is an important mediator of inflammatory responses. Researchers use IL-6 detection systems to investigate cytokine production and inflammatory mechanisms.

2. Immunology Studies

Supports research into immune-cell activation, cytokine networks, and interactions between innate and adaptive immunity.

3. Autoimmune Disease Research

IL-6 signaling is investigated in autoimmune and inflammatory disorders, including rheumatoid arthritis, systemic lupus erythematosus, and multiple sclerosis.

4. Infectious Disease Research

IL-6 production is studied during immune responses to bacterial and viral infections, including investigations of cytokine responses during severe respiratory infections.

5. Cancer Research

IL-6 participates in signaling networks associated with tumor inflammation, cell survival, proliferation, and interactions between tumor cells and the immune microenvironment.

6. Cardiovascular and Metabolic Research

IL-6 is investigated in relation to atherosclerosis, metabolic regulation, and inflammatory processes associated with cardiovascular and metabolic disorders.

Technical Specifications

Handling and Storage Recommendations

• Follow the manufacturer's datasheet for the recommended storage temperature.
• Avoid repeated freeze-thaw cycles where applicable.
• Use clean laboratory equipment and appropriate pipetting techniques.
• Prepare assay standards and samples according to the validated ELISA protocol.
• Use the recommended detection antibody for sandwich ELISA experiments.
• Include appropriate positive, negative, and blank controls.
• Optimize antibody concentrations and incubation conditions according to the assay protocol.

Frequently Asked Questions

1. What does the Human IL-6 antibody detect?

It recognizes human Interleukin-6 (IL-6), a multifunctional cytokine involved in inflammation and immune regulation.

2. What is the function of this antibody in ELISA?

It serves as the capture antibody in a sandwich ELISA, immobilizing human IL-6 from biological samples for subsequent detection.

3. What does BSA and Azide Free mean?

It means that the antibody formulation is supplied without bovine serum albumin (BSA) and sodium azide.

4. Which detection antibody should be used with HA721167?

The recommended detection antibody is HA721168, supplied in an unconjugated format.

5. What is the reported IL-6 assay range?

The reported reference range for the recommended human IL-6 sandwich ELISA system is 5–1000 pg/mL.

6. What is the supplied antibody volume?

The product is supplied in a 100 µL vial.